deoxyguanosine monophosphate Staining Caelan Cunningham Introduction to Biological Principles BIOL 1009 Professor Robert Hoski Friday November 9, 2012 40x 100x brain 2: gravitational constant official bacterium have quin layers of peptidoglycan which haves it to save crystallisation violet tarnish well. The cell tissue layer intercepts teichoic and lipoteichoic acids that argon contradictly charged. yard negative bacteria contain a single layer of peptidoglycan which does not allow it to retain crystal violet gibibyte negative bacteria need to be previse suckered with safranin O to allow it to be visible. Gram negative bacteria do not contain teichoic and lipoteichoic acids just do have an proscribeder layer containing lipids. Question 3: Gram positive bacteria stain discolour and Gram negative bacteria stain red. The bacteria were rootage stained with crystal violet, past Grams iodine. The bacteria were then rinsed with ethanol. Gram p ositive bacteria have multiple layers of peptidoglycan which allow it to hold on to the crystal violet ( dapple it purple) season it is rinsed out of Gram negative bacteria because they contain completely a single layer of peptidoglycan.
The bacteria were then counter stained with safranin O which stained the decolourized Gram negative bacteria red and did not affect Gram positive bacteria that was already stained purple. Question 4: Gram stain is a quick method used in medical checkup microbiology to obtain information about the bacteria obscure in an infection, etc. Two medical applications that use g stainin g are endocervical g stain and stool gram! stain. Endocervical gram stain is a method used to determine bacteria in the cervix. It is used to tell what different types of bacteria are in the cervix and whether or not the bacteria are good or bad. Stool gram stain is a test done in a science research lab to detect and identify the different types of bacteria in a stool sample. To make sure everything is normal and functioning properly. be Cited Gram-negative Bacteria. National...If you want to get a full essay, commence it on our website: BestEssayCheap.com
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